Apoptosis is the most common form of programmed cell death and is a central and complex process for embryonic development and maintenance of cell homeostasis in multicellular organisms. We applied different quantitative proteomics approaches to cells exposed to chemotherapeutic drugs (Cisplatin, Sorafenib, STLC, and Taxol) to find proteins involved in the apoptotic process.
A particular focus was on temporal proteome profiling and analyses of protein complexes (e.g., lipid rafts and the proteasome). For relative proteome quantification, we have recently establihed isobaric peptide termini labelling (IPTL). The IPTL approach is based on crosswise chemical labelling of both peptide termini with complementary isotopically labelled reagents. As a result, all N- and C-terminal fragment ions will provide quantification data for each peptide throughout the MS/MS spectrum.
Prof. Bernd Thiede is a group leader at the Biotechnology Centre of Oslo. He studied chemistry in Hamburg. He went for his Ph.D. at the Max Delbrück Center for Molecular Medicine (MDC) in Berlin, then he spent five years at the Max Planck Institute for Infection Biology in Berlin and at the RCSI in Dublin before he moved to the Biotechnology Centre of Oslo. There, he investigates the differences of the proteomes of drug-treated apoptotic cells and untreated cells.
Moderation: Dr. Frank Schmidt